Week of flow cytometry courses aimed at both beginners & for those whom flow cytometry is a main tool. Can be taken as individual or a block

Courses can be taken as individual course or a block.

Monday 5th October - Introduction to Flow Cytometry: The Foundation Block is aimed at those new to flow cytometry and those wanting to understand the basics of the technology before attending further Building Blocks. This can be taken as a standalone one day course or can be taken as a building block for the next courses. The course starts with an introduction to this quickly evolving technology, its importance and resources that are available. One of the main aims of this course is to give the delegate a firm understanding of fluorescence, fluorochromes and the workings of a cytometer. This is followed by talks on fluorescence compensation, sample preparation, basics of experimental design, data analysis and reporting your flow cytometry data.

Tuesday 6th October - Advanced Flow Cytometry: This interactive course builds on the Introductory course and goes into greater details on how a cytometer works, best practices for running cytometry experiments, experimental design, panel design, compensation and data spreading and presentation of data. As cytometers become capable of measuring an increasing number of fluorochromes, experimental design becomes more complicated. It requires knowledge of the fluorochrome, the biology (antigen density, co-expression) and the sample preparation as well as as a greater understanding about the cytometer. In this one-day course we will look at the factors that need to be taken into account to build multicolour panels. We will do several interactive panel designs where we will use this knowledge to design panels of 6-10 and discuss panels with larger numbers of fluorochromes.

Wednesday 7th October - Practical Data Analysis: Data analysis of flow data files can be daunting. Where do I set regions and gates? Which metric do I use? In this one-day course we will guide delegates through the minefield of data analysis from simple gating to high dimensional analysis. This one-day course will introduce delegates to the analysis of flow data. We will cover the plot types, setting compensation, regions and gates, the metrics that can be derived, and data presentation. We will illustrate all these with data files that will be available to delegates.

PLEASE NOTE: YOU ARE REQUIRED TO BRING A LAPTOP TO THE DATA COURSE AND PLEASE DOWNLOAD THE LATEST VERSION OF FLOWJO ON TO IT. WILL WE SEND DETAILS OF A TEMPORARY FLOWJO LICENCE TO BE USED ON THE COURSE NEARER THE TIME OF THE COURSE. IF YOU DO NOT HAVE A LAPTOP PLEASE LET US KNOW IN ADVANCE AND WE WILL BE ABLE TO PROVIDE ONE.

Thursday 8th October - Practical Spectral Flow Cytometry : The Spectral Flow Cytometry Building Block is aimed at those new to Spectral flow cytometry and those wanting to understand the basics of the technology before attending further Building Blocks. This can be taken as a standalone one day course or can be taken as a building block for the next courses. The course starts with an introduction to Spectral Flow Cytometry Technologies, its importance and why it is become a key tool for scientists.

This one-day course will combine theory and practical. Delegates will learn what spectral flowcytometry can bring to their experiments. We will look at how Spectral flow cytometers differ from conventional cytometers in their light detection, how this influences experimental design and some of the specific considerations of using this approach. We will run a simple experiment on spectral cytometers - the Cytek Aurora, BD FACSDiscover S8 and the Sony ID7000.

Friday9th October - Practical Imaging Cytometry : . The course can be taken as a standalone one day course or can be taken as a building block to add to your knowledge from our other flow cytometry courses. The course starts with an introduction to Imaging Cytometry, including information about many of the commercially avalaible image cytometers. There will be practicals in Babraham Institute Flow Cytometry Facility on the Thermo Attune CytPix, Cytek Amnis Imagestream MkII and BD FACSDiscover S8 spectral cell sorter with imaging capabilities. The day will will conclude with sessions on data analysis from these systems and an overview of presentation of imaging data.

The in person courses includes refreshments, including lunch, morning and afternoon tea and coffee and course notes sent in advance.

Discounts available:

50% off for academics use the Code Academic50

10% off for companies on Babraham Research Campus or use Babraham Institute Flow Facility use the code BRC10

20% off for a company person booking on to multiple courses or multiple people from same company booking on to the course use the code MULTI20

Description supplied by the event organiser.